Journal: Journal of Inflammation (London, England)

Article Title: Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver

doi: 10.1186/1476-9255-2-6

Figure Lengend Snippet: IL-6 protein production in sera following parthenolide and LPS co-treatment. Female B6C3F1 mice were co-treated with parthenolide (5 mg/kg, i.p.) or 50 μl DMSO and LPS (1 mg/kg, i.p.) or 100 μl water. After 90 minutes, blood was collected and serum analyzed for IL-6 by ELISA. The letter (a) indicates a significant difference compared to vehicle and parthenolide controls; (b) indicates a significant difference compared to LPS. Data are mean ± SEM (n = 16, controls n = 4), and is a combination of 4 separate experiments.

Article Snippet: IL-6 analysis was performed using purified and biotin-conjugated rat anti-mouse IL-6 antibodies from PharMingen (San Diego, CA) as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Inflammation (London, England)

Article Title: Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver

doi: 10.1186/1476-9255-2-6

Figure Lengend Snippet: IL-6 mRNA expression levels in spleen and liver following parthenolide and LPS co-treatment. Female B6C3F1 mice were co-treated with parthenolide (5 mg/kg, i.p.) or 50 μl DMSO and LPS (1 mg/kg, i.p.) or 100 μl water. Spleen and liver were collected after 90 minutes and total RNA was extracted and subjected to real-time, one-step RT-PCR using TaqMan primers and probes. IL-6 mRNA levels were normalized using 18S rRNA and related to spleen control values. (a) indicates a significant difference compared to vehicle and parthenolide controls; (b) indicates a significant difference compared to LPS. Data are mean ± SEM (n = 16, controls n = 4), and is a combination of 4 separate experiments.

Article Snippet: IL-6 analysis was performed using purified and biotin-conjugated rat anti-mouse IL-6 antibodies from PharMingen (San Diego, CA) as described previously [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Schematic illustration of ( a ) the synthesis of bio-functionalized core-shell SERS nanotag; ( b ) the detection of IL-6 by core-shell SERS nanotags combined with the IFA kit.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques:

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Optimization of the SERS IFA conditions: ( a ) optimization of the amount of labelled antibody in the preparation of Au 4-MBA @Ag@Ab; ( b ) optimization of the amount of Au 4-MBA @Ag@Ab solution; ( c ) optimization of coating IL-6 antibody concentration on the NC membrane; ( d ) optimization of different washing times.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Concentration Assay

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: ( a ) Image of the test dot (4 mm in diameter) for different concentrations of IL-6; ( b ) averaged Raman spectra of test dot for different concentrations of IL-6 (A-H corresponding to 20 fg mL 〓1 -200 ng mL −1 ); ( c ) the calibration curve of IL-6, and the inset shown the linear part. The error bars are calculated from three measurements.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques:

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Specificity of SERS IFA. ( a ) SERS spectra of Au 4-MBA @Ag at 1076 cm −1 , and ( b ) Raman intensity for IL-6, IL-8, IL-10, CRP, and SAA at a concentration of 100 pg mL −1 .

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Concentration Assay

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: ( a ) Western blot analysis of IL-6 protein spiked in the milk; ( b ) SERS spectra of different concentrations (0 ng mL –1 -1 µg mL −1 ) of IL-6 spiked in the milk and ( c ) the linear calibration curve; ( d ) comparison of detection results obtained from proposed method (red circles) and reference ECLIA method (black squares). Error bars are calculated from three measurements.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Western Blot